Affymetrix Array Facility

Headed by Professor Bryan D Young

Affymetrix GeneChip arrays are high-density oligonucleotide microarrays manufactured using a photolithographic technology. This system allows you to measure the relative concentration of an RNA or DNA sequence in a complex mixture of nucleic acids. Expression arrays enable researchers to simultaneously monitor genome-wide expression profiles, while SNP Mapping Arrays enable large-scale linkage analysis, association, and copy number studies.

The Array Facility at Charterhouse Square, was established from a grant to Prof B Young from The Special Trustees and consists of a complete Affymetrix GeneChip System comprising two Fluidics 450 Wash Stations, two Hybridisation Ovens and a GeneChip Scanner 3000 7G. The equipment allows for efficient and concurrent throughput of both Gene Expression and SNP Genotyping chips.

A chip hybridization service is provided for the College and Cancer Institute at Charterhouse Square. Advice is offered on experimental design, probe making and chip ordering.

A 'flexible' booking system is operated and you are expected to provide your own chips and sample cocktail. Experimental data is provided on CD for your analysis.

You are encouraged to track the quality of your probe using our Agilent 2100 Bioanalyser which utilizes a "Lab-on-a-Chip" system to assess total RNA and fragmented cRNA quality.  

Contact Details

Professor Bryan Young
Run by:
Tracy Chaplin
Senior Scientific Officer
Room 311, Med Oncology Laboratory, John Vane Science Centre, Charterhouse Square
Tel 020 7882 5612

Recent publications

Young, B. D., S. Debernardi, et al. (2006). "A role for mitotic recombination in leukemogenesis." Adv Enzyme Regul 46: 90-7.

Strefford, J. C., F. W. van Delft, et al. (2006). "Complex genomic alterations and gene expression in acute lymphoblastic leukemia with intrachromosomal amplification of chromosome 21." Proc Natl Acad Sci U S A 103(21): 8167-72.

Strefford, J. C., T. M. Lane, et al. (2006). "Molecular characterisation of the t(1;15)(p22;q22) translocation in the prostate cancer cell line LNCaP." Cytogenet Genome Res 112(1-2): 45-52.

Dunne, J., C. Cullmann, et al. (2006). "siRNA-mediated AML1/MTG8 depletion affects differentiation and proliferation-associated gene expression in t(8;21)-positive cell lines and primary AML blasts." Oncogene 25(45): 6067-78.

Bungaro, S., M. Raghavan, et al. (2006). "Assessment of submicroscopic genetic lesions by single nucleotide polymorphism arrays in a child with acute myeloid leukemia and FLT3-internal tandem duplication." Haematologica 91(7): 998-1000.

Wilson, C., J. Yang, et al. (2005). "Overexpression of genes on 16q associated with cisplatin resistance of testicular germ cell tumor cell lines." Genes Chromosomes Cancer 43(2): 211-6.

Teh, M. T., D. Blaydon, et al. (2005). "Genomewide single nucleotide polymorphism microarray mapping in basal cell carcinomas unveils uniparental disomy as a key somatic event." Cancer Res 65(19): 8597-603.

Strefford, J. C., I. Stasevich, et al. (2005). "A combination of molecular cytogenetic analyses reveals complex genetic alterations in conventional renal cell carcinoma." Cancer Genet Cytogenet 159(1): 1-9.

Raghavan, M., D. M. Lillington, et al. (2005). "Genome-wide single nucleotide polymorphism analysis reveals frequent partial uniparental disomy due to somatic recombination in acute myeloid leukemias." Cancer Res 65(2): 375-8.

Lu, Y. J., J. Yang, et al. (2005). "Association between large-scale genomic homozygosity without chromosomal loss and nonseminomatous germ cell tumor development." Cancer Res 65(20): 9137-41.

Kelsell, D. P., E. E. Norgett, et al. (2005). "Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis." Am J Hum Genet 76(5): 794-803.

Fitzgibbon, J., L. L. Smith, et al. (2005). "Association between acquired uniparental disomy and homozygous gene mutation in acute myeloid leukemias." Cancer Res 65(20): 9152-4.

Woodfine, K., H. Fiegler, et al. (2004). "Replication timing of the human genome." Hum Mol Genet 13(2): 191-202.

Mao, X., G. Orchard, et al. (2004). "BCL2 and JUNB abnormalities in primary cutaneous lymphomas." Br J Dermatol 151(3): 546-56.

Liu, W. M., S. J. Strauss, et al. (2004). "s-thalidomide has a greater effect on apoptosis than angiogenesis in a multiple myeloma cell line." Hematol J 5(3): 247-54.

Debernardi, S., D. Lillington, et al. (2004). "Understanding cancer at the chromosome level: 40 years of progress." Eur J Cancer 40(13): 1960-7.

Lillington, D. M., J. E. Kingston, et al. (2003). "Comparative genomic hybridization of 49 primary retinoblastoma tumors identifies chromosomal regions associated with histopathology, progression, and patient outcome." Genes Chromosomes Cancer 36(2): 121-8.

Debernardi, S., D. M. Lillington, et al. (2003). "Genome-wide analysis of acute myeloid leukemia with normal karyotype reveals a unique pattern of homeobox gene expression distinct from those with translocation-mediated fusion events." Genes Chromosomes Cancer 37(2): 149-58.

Chambers, A. E., S. Banerjee, et al. (2003). "Histone acetylation-mediated regulation of genes in leukaemic cells." Eur J Cancer 39(8): 1165-75.